期刊
ANALYTICAL BIOCHEMISTRY
卷 336, 期 2, 页码 305-311出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2004.10.027
关键词
direct electron transfer; mouse plasma cancer; multiwalled carbon nanotubes; putrescine biosensor
Poly(diallyldimethylammonium) chloride, having a capability of dispersing multiwalled carbon nanotubes (MWCNTs), permits the modification of electrode surfaces. Together with putrescine oxidase, a MWCNT modified glassy carbon electrode was constructed for the development of a mediatorless putrescine biosensor. Nanoscale dendrites of MWCNTs were reasoned to form a network, projecting outward from the electrode surface acting like bundled ultra-microelectrodes, thereby permitting access to the active site and facilitating direct electron transfer to the immobilized enzyme. Our biosensor was capable of efficiently monitoring the direct electroactivity of putrescine oxidase at the electrode surface. Direct electron transfer permits the detection of putrescine at negative potentials, circumventing the interference of endogenous ascorbic and uric acids, which often complicate the analysis of important compounds in plasma. Compared with the most common interfering species, such as spermine, spermidine, cadaverine, and histamine. a detection limit of 5 muM and a response 20 times greater were found for putrescine. Tests performed on plasma of cancerous mice demonstrated that the detection of putrescine could be carried out very quickly on mammalian plasma without previous purification. (C) 2004 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据