期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 3, 页码 530-535出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0408568102
关键词
conjugated polymers; DNA sensors; time-resolved anisotropy; ultrafast spectroscopy
资金
- NIGMS NIH HHS [R01 GM062958, GM62958-01] Funding Source: Medline
The two-step energy-transfer process in a self-assembled complex comprising a cationic conjugated polymer (CCP) and a dsDNA is investigated by using pump-clump-emission spectroscopy and time-correlated single-photon counting; energy is transferred from the CCP to an ethiclium bromide (EB) molecule intercalated into the dsDNA through a fluorescein molecule linked to one terminus of the DNA. Time-dependent anisotropy measurements indicate that the inefficient direct energy transfer from the CCP to the intercalated EB results from the near orthogonality of their transition moments. These measurements also show that the transition moment of the fluorescein spans a range of angular distributions and lies between that of the CCP and EB. Consequently, the fluorescein acts as a fluorescence resonance energy-transfer gate to relay the excitation energy from the CCP to the EB.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据