期刊
FEBS LETTERS
卷 579, 期 3, 页码 655-660出版社
WILEY
DOI: 10.1016/j.febslet.2004.12.037
关键词
actin; malaria; cytoskeleton dynamics; DNasel; phalloidin; Plasmodium falciparum
Plasmodium falciparum, the etiologic agent of malaria, is a facultative intracellular parasite of the phylum Apicomplexa. A limited turnover of microfilaments takes place beneath the parasite plasma membrane, but the cytoplasm of api-complexans is virtually devoid of F-actin. We produced Plasmodium actin in yeast. Purified recombinant Plasmodium actin polymerized inefficiently unless both gelsolin and phalloidin were added. The resulting actin polymers appeared fragmented in the fluorescence microscope. Plasmodium actin bound DNaseI about 200 times weaker than bovine non-muscle actin. Our findings suggest that the unique properties of Plasmodium actin can explain some of the unusual features of apicomplexan parasite microfilaments. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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