Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands

Manganese lipoxygenase is secreted by the fungus Gaeumannomyces graminis. We expressed the enzyme in Pichia pastoris, which secreted similar to30mg Mn-lipoxygenase/L culture medium in fermentor. The recombinant lipoxygenase was N- and O-glycosylated (80100 kDa), contained similar to1 mol Mn/mol protein, and had similar kinetic properties (K-m similar to7.1 muM alpha-linolenic acid and V-max 18 nmol/min/mug) as the native Mn-lipoxygenase. Mn-lipoxygenase could be quantitatively converted, presumably by secreted Pichia proteases, to a smaller protein (similar to67 kDa) with retention of lipoxygenase activity (K-m similar to6.4 muM alpha-linolenic acid and V-max similar to12 nmol/min/mug). Putative manganese ligands were investigated by site-directed mutagenesis. The iron ligands of soybean lipoxygenase-1 are two His residues in the sequence HWLNTH, one His residue and a distant Asn residue in the sequence HAAVNFGQ, and the C-terminal Ile residue. The homologous sequences of Mn-lipoxygenase are (HVLFH278)-V-274 and H(462)HVMN(466)QGS, respectively, and the C-terminal amino acid is Val-602. The His274Gln, His278Glu, His462Glu, and the Val-602 deletion mutants of Mn-lipoxygenase were inactive, and had lost >95%, of the manganese content. His-463, Asn-466, and Gln-467 did not appear to be critical for Mn-lipoxygenase activity, as His463Gln, Asn466Gln, Asn466Leu, and Gln467Asn mutants metabolized alpha-linolenic acid to 11- and 13-hydroperoxylinolenic acids. We conclude that His-274, His-278, His-462, and Val-602 likely coordinate manganese. (C) 2004 Elsevier Inc. All rights reserved.