期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 61, 期 4, 页码 755-760出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2004.05.038
关键词
surface-enhanced Raman scattering; SERS; imaging; single cells; lymphocyte; rhodamine 6G; principal component analysis; PCA; multivariate analysis
类别
This paper is aimed to show the possibility to determine individual organic compounds introduced into single living cells with surface-enhanced Raman spectroscopy (SERS). Surface enhancement was achieved with gold colloids that were allowed to diffuse into lymphocytes. An introduced analyte, rhodamine 6G, could be imaged together with for example nucleotides and amino acids of the cell. Multivariate evaluation of surface-enhanced Raman images proved to be a powerful tool for the separation of spectral information of various intracellular components. The principal component analysis (PCA) enabled identification of spectra containing different chemical information and separation of the spectral contribution of rhodamine 6G from the complex cellular matrix. (C) 2004 Elsevier B.V. All rights reserved.
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