Thermostable cellulases from Streptomyces sp.:: scale-up production in a 50-l fermenter

Thermostable cellulase was produced by Streptomyces sp. T3-1 grown in a 50-1 fermenter. Maximum cellulase activity was attained on the fourth day when agitation speeds and aeration rates were controlled at 300 rpm and 0.75 vvm, respectively. Maximum enzyme activities were: 148 IU CMCase ml(-1), 45 IU Avicelase ml(-1), and 137 IU beta-glucosidase ml(-1) with productivity of 326 IU l(-1) h(-1), which were 10-32% higher than the values obtained in shake-flask cultures.