期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 100, 期 1, 页码 43-46出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762005000100008
关键词
cutaneous leishmaniasis; sand flies vectors; natural infections; polymerase chain reaction; Lutzomyia spp.; Venezuela
We studied the natural infection of Lutzomyia (Lutzomyia) sp. with Leishmania in endemic foci of cutaneous leishmaniasis in the Paria peninsula, state of Sucre, Venezuela. Sandflies were collected between March 2001 and June 2003, using Shannon light-traps and human bait. Of the 1291 insects captured, only two species of phlebotomines were identified: L. ovallesi (82.75%) and L. gomezi (17.42%). A sample of the collected sand flies (51 pools of 2-12 individuals) were analyzed by using a multiplex-PCR assay for simultaneous detection of few Word Leishmania and Viannia subgenera. The results showed a total of 8 pools (15.68%) infected; of these, 7 were L. ovallesi naturally infected with L. braziliensis (2 pools) and L. mexicana (5 pools) and 1 pool of L. gomezi infected by L. braziliensis.
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