4.4 Article

Increased noxl and hydrogen peroxide in prostate cancer

期刊

PROSTATE
卷 62, 期 2, 页码 200-207

出版社

WILEY
DOI: 10.1002/pros.20137

关键词

prostate; cancer; reactive; oxygen; Nox1

资金

  1. NCI NIH HHS [CA84138, CA098912] Funding Source: Medline

向作者/读者索取更多资源

BACKGROUND. Reactive oxygen species (ROS) are emerging as candidate mediators of growth and angiogenesis in cancer. Increased ROS often correlates with cell growth, e.g., Ras-transformed cells and cells treated with growth factors. While non-transformed cells respond to growth factors/cytokines with the regulated production of ROS, tumor cells in culture frequently overproduce H2O2. We propose that NADPH oxidases (Nox) account for increased levels of ROS in some cancers. Previously, transfection of Nox1 into a prostate cancer cell line dramatically enhanced tumor growth (Arbiser et al.: PNAS 99:715-720, 2001). METHODS. Using immunohistochemistry, immunofluorescence, dihydroethidium staining, and Flow cytometry, we investigated the correlation between Nox1 and ROS in prostate cancer. RESULTS. Here, we demonstrate that human prostate tumors show increased H2O2 levels. Furthermore, 80% of human prostate tumor samples show markedly increased Nox1 protein levels and increased mRNA levels. In addition, a series of cell lines developed from LNCaP prostate cancer cells that demonstrate increasing tumor and metastatic potential, show increased Nox1 and a parallel increase in H2O2 levels. CONCLUSIONS. The results illustrate that human prostate cancer frequently show both increased H2O2 and Nox1, and that in an animal model system increased Nox1/H2O2 correlates with increased tumorigenicity. (C) 2004 Wiley-Liss, Inc.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据