期刊
PROTEIN AND PEPTIDE LETTERS
卷 12, 期 2, 页码 207-210出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/0929866053005854
关键词
reversible immobilization; surface modification; microreactor; enzyme reaction
We developed a simple immobilisation method for His-tagged enzymes on a microchannel surface. It facilitates immobilisation of protein molecule on microchannel surface through Ni-complex, using crude or purified protein solutions. By this method, we could immobilize proteins on microcapillary constantly. This method might be useful for further development of microreactor with reversibly immobilized enzymes.
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