期刊
JOURNAL OF NEUROCHEMISTRY
卷 92, 期 4, 页码 780-789出版社
WILEY
DOI: 10.1111/j.1471-4159.2004.02907.x
关键词
actin; chromaffin; cell; exocytosis; scinderin
Stimulation-induced chromaffin cell cortical F-actin disassembly allows the movement of vesicles towards exocytotic sites. Scinderin (Sc), a Ca2+-dependent protein, controls actin dynamics. Sc six domains have three actin, two PIP2 and two Ca2+-binding sites. F-actin severing activity of Sc is Ca2+-dependent, whereas Sc-evoked actin nucleation is Ca2+-independent. Sc domain role in secretion was studied by co-transfection of human growth hormone (hGH) reporter gene and green fluorescent protein (GFP)-fusion Sc constructs. Cells over-expressing actin severing Sc1-6 or Sc1-2 (first and second actin binding sites) constructs, increased F-actin disassembly and hGH release upon depolarization. Over-expression of nucleating Sc5-6, Sc5 or ScABP(3) (third actin site) constructs decreased F-actin disassembly and hGH release upon stimulation. Over-expression of ScL5-6 or ScL5 (lack of third actin site) produced no changes. During secretion, actin sites 1 and 2 are involved in F-actin severing, whereas site 3 is responsible for nucleation (polymerization). Sc functions as a molecular switch in the control of actin (disassembly <----> assembly) and release (facilitation <----> inhibition). The position of the switch (severing <----> nucleation) may be controlled by [Ca2+](i). Thus, increase in [Ca2+](i) produced by stimulation-induced Ca2+ entry would increase Sc-evoked cortical F-actin disassembly. Decrease in [Ca2+](i) by either organelle sequestration or cell extrusion would favor Sc-evoked actin nucleation.
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