期刊
GENES BRAIN AND BEHAVIOR
卷 4, 期 1, 页码 51-59出版社
WILEY
DOI: 10.1111/j.1601-183X.2004.00098.x
关键词
aryl hydrocarbon receptor; hippocampus; mossy fibers; radial arm maze; TCDD
资金
- NIEHS NIH HHS [ES01332] Funding Source: Medline
Perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has been reported to alter spatial learning in rats tested on a radial arm maze (RAM). TCDD is believed to exert most of its effects through binding to the aryl hydrocarbon receptor (AhR). To determine whether the AhR mediates TCDD-induced alterations in spatial learning, we tested male and female AhR-knockout (AhR(-/-)), heterozygous (AhR(+/-)) and wild-type (AhR(+/+)) mice on the RAM. AhR(+/-) male and female mice were time mated, and treated dams were dosed with 5 mug TCDD/kg body weight on day 13 of gestation. When offspring reached adulthood, male and female AhR(+/+), AhR(+/-) and AhR(-/-) mice from TCDD-exposed and unexposed litters were tested on the eight-arm RAM. After testing, we examined hippocampal morphology as visualized by the Timm's silver sulfide stain. TCDD-exposed female AhR(+/-) mice made more errors than their respective controls on the RAM and exhibited a decrease in the size of the intra- and infrapyramidal mossy fiber (IIP-MF) field of the hippocampus. None of the other TCDD-exposed groups differed from their respective control groups with regard to maze performance or hippocampal morphology. The reduction of IIP-MF field indicates a possible morphological basis for the learning deficit that was observed in the female AhR(+/-) mice. It is hypothesized that the effect of TCDD exposure is AhR dependent and that TCDD may alter GABAergic activity in the hippocampus of female mice during development.
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