期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 56, 期 412, 页码 597-603出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eri050
关键词
ABI5; bZIP transcription factors; multigene family; RNA interference; seed maturation
The Arabidopsis thaliana genome contains approximately 80 genes encoding basic leucine zipper transcription factors, divided into 11 groups. Abscisic Acid-Insensitive 5 (AB15) is one of the 13 members of group A and is involved in ABA signalling during seed maturation, and germination. Seven other members of this group are expressed during seed maturation, but only one of them (Enhanced Em Level, EEL) has been functionally characterized during this developmental phase. Since EEL and two other group A genes, AtbZIP67 and AREB3 (ABA-Responsive Element Binding protein 3), display similar mRNA temporal expression in whole siliques, it is suspected that they might share some overlapping functions. To address this question, the proteins' tissular and subcellular localization in transgenic Arabidopsis were precisely characterized, using translational fusions with a green fluorescent protein (GFP) expressed under the corresponding bZIP promoter. It was found that the three fusion proteins were expressed with a largely overlapping pattern and constitutively localized in the nuclei. An RNA interference approach (RNAi) was then used to knock out the expression of all three genes simultaneously. Endogenous EEL, AREB3, and AtbZIP67 transcripts could be specifically reduced, but no visible defects could be observed during seed maturation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据