期刊
FEMS MICROBIOLOGY LETTERS
卷 243, 期 1, 页码 271-278出版社
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2004.12.012
关键词
Pseudomonas aeruginosa; real time RT-PCR; virulence genes; experimental lung infection
类别
The bacterial aspects of the events occurring during the first days of lung colonization by Pseudomonas aeruginosa are still unknown. The aim of this study was to develop a real time RT-PCR method for the direct quantification of the transcripts of three P. aeruginosa virulence genes: exoS, lasl and algD, during the first seven days of a rat lung infection. Our results document differences in bacterial gene expression throughout P. aerliginosa infection. E-xoS transcripts levels were very high in the first days of infection, but a significant decrease was then progressively observed. Transcription of algD occurred on the 4th day and increased regularly over time suggesting a balance in the transcription of exoS and algD. The strong expression of exoS during the first 3 days was correlated to 29% of mortality among infected rats. On the contrary, the increase of algD expression after this period was associated to the development of a chronic infection with no further mortality. LasI transcription remained more constant throughout the infection. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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