期刊
FEMS MICROBIOLOGY LETTERS
卷 243, 期 1, 页码 259-263出版社
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2004.12.014
关键词
Shigella; enteroinvasive; Escherichia coli; LAMP; ipaH gene detection
类别
Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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