期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 43, 期 2, 页码 125-131出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2005.01.006
关键词
Capsicum frutescens; normal roots; biotransformation; caffeic acid; veratraldehyde; methyl jasmonate; caffeic acid O-methyl transferase
Normal root cultures of Capsicum frutescens biotransform externally fed precursors, like caffeic acid and veratraldehyde, to vanillin and other related metabolites. The bioconversion of caffeic acid to further metabolites-viz. vanillin, vanillylamine, vanillic acid-was shown to be elicited by treating the cultures with 10 mu M methyl jasmonate (W). Root cultures treated with MJ accumulated (1.93 times) more of vanillin (20.2 mu M on day-3) than untreated ones. A concomitant increase in enzymatic activity of caffeic acid O-methyl transferase (CAOMT, EC 2.1.1.68) was obtained in MJ treated cultures, compared to untreated cultures. After 24 h of MJ treatment, a 13.7-fold increase in CAOMT activity was recorded in root cultures of C. frutescens. Cultures treated with veratraldehyde accumulated more vanillin (78 mu M) than caffeic acid fed cultures, 6 days after precursor addition. Capsaicin did not accumulate even after addition of precursors. The efficiencies of biotransformation with caffeic acid and veratraldehyde were 2.2% and 9% with respect to vanillin formation, indicating a possible diversion of the phenylpropanoid pathway towards other secondary metabolites. (c) 2005 Elsevier SAS. All rights reserved.
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