期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 562, 期 3, 页码 785-799出版社
WILEY
DOI: 10.1113/jphysiol.2004.079228
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资金
- NIDCD NIH HHS [R01 DC002994-10, DC 02994, R01 DC002994, DC 04953] Funding Source: Medline
- NINDS NIH HHS [NS 07938] Funding Source: Medline
The electrical properties of sustentacular cells (SCs) in the olfactory epithelium (OE) were investigated in tissue slices taken from neonatal mice (P0-P4). Conventional whole-cell recordings were obtained from SCs and also from olfactory receptor neurones (ORNs) in situ. SCs had a larger apparent cell capacitance (C-cell) (18.6 +/- 0.5 pF) than ORNs (4.4 +/- 0.4 pF) and a lower apparent membrane resistance (R-m) (160 +/- 11 MOmega versus 664 +/- 195 MOmega, respectively). When corrected for a seal resistance of 1 GOmega, these mean R-m values were increased to 190 MOmega and 2 GOmega in SCs and ORNs, respectively. SCs generated a TTX (1mum)-resistant voltage-activated Na+ current (I-Na) that had a peak density at -38 mV of -44 pA pF(-1) and supported action potential firing. Peak current density of I-Na in neurones was 510 +/- 96 pA pF(-1). The outward K+ current in SCs was composed (> 70%) of a TEA (2 mm)-sensitive component that was mediated by the opening of large-conductance (237 +/- 10 pS; BK) channels. The resting leak conductance (90 of SCs was permeable to monovalent cations and anions and was largely inhibited by substitution of external Na+ with NMDG and by internal F- with gluconate. g(L) deactivated up to 50% at potentials negative of -70 mV and was inhibited by 18beta-glycyrrhetinic acid (20 mum). SCs were identified using fluorescent dyes (Lucifer Yellow and Alexa Fluor 488) in the whole-cell patch pipette-filling solution. Our findings indicate that SCs in the OE of neonates are electrically excitable and are distinguishable from neurones by a having a resting g(L).
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