期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 381, 期 4, 页码 948-952出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-004-2994-2
关键词
polypyrrole; stainless steel frit; micro solid phase extraction; ochratoxin A; pulsed elution; high performance liquid chromatography
Polypyrrole (PPy) was electrochemically synthesized on stainless steel frits as a sorbent for the micro solid phase extraction (muSPE) of ochratoxin A (OTA). Using 20 mul of standard solution under a fast flow rate of 0.5 ml/min, 80% recovery of OTA was achieved in the concentration range from 0.1-10 pg/mul. This good recovery was achieved within a short residence time of 1.2 s. A binding capacity of 1 ng OTA was estimated for each PPy-modified frit, or 2 ng OTA for two frits in series. The bound OTA could be pulsed eluted (PE) with 20 mul of 1% triethylamine in acetonitrile. On-line coupling of this PPy-on-a-frit and PE technique to high performance liquid chromatography (HPLC) was straightforward. On-line muSPE-PE-HPLC results clearly demonstrated the capability of PPy-on-a-frit to bind OTA in the presence of red wine, beer, and orange juice components.
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