期刊
BIOTECHNOLOGY LETTERS
卷 27, 期 4, 页码 259-263出版社
SPRINGER
DOI: 10.1007/s10529-004-8295-1
关键词
biofuel; cellobiose; beta-glucosidase; metabolic engineering; Zymomonas mobilis
To confer the ability to ferment cello-oligosaccharides on the ethanol-producing bacterium, Zymomonas mobilis, the beta-glucosidase gene from Ruminococcus albus, tagged at its N-terminal with the 53-amino acid Tat signal peptide from the periplasmic enzyme glucose-fructose oxidoreductase from Z. mobilis, was introduced into the strain. The tag enabled 61% of the beta-glucosidase activity to be transported through the cytoplasmic membrane of the recombinant strain which then produced 0.49 g ethanol/g cellobiose.
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