期刊
PROTEOMICS
卷 5, 期 2, 页码 541-550出版社
WILEY
DOI: 10.1002/pmic.200400934
关键词
cerebrospinal fluid; mass spectrometry; prefractionation; solid-phase extraction; two-dimensional gel electrophoresis
资金
- NCRR NIH HHS [RR 14953, RR 10522] Funding Source: Medline
- NIDA NIH HHS [DA 12212] Funding Source: Medline
Cerebrospinal fluid (CSF) is produced by the chorioid plexus in the ventricles. It surrounds the Received: March 3, 2004 brain and bone marrow, and reflects several different disorders of the central nervous system (CNS). Revised: June 11, 2004 Proteomics has been used to analyze CSF in order to discover disease-associated proteins and to Accepted: June 18, 2004 elucidate the basic molecular mechanisms that either cause, or result from, CNS disorders. However, some disease-associated proteins are of low-abundance and are difficult to detect. A low total-protein concentration, a high amount of albumin and immunoglobins, and a wide dynamic range (several orders of magnitude) of protein concentration cause several difficulties in the identification of low-abundance C S F proteins. In this study, advantage was taken of the range of different hydrophobic properties of CSF proteins, and a reversed-phase solid-phase extraction (SPE) cartridge was used to prefractionate human lumbar CSF proteins into three separate fractions prior to two-dimensional gel electrophoresis resolution of the proteome. A portion of the high-abundance CSF proteins were removed from two (eluted with 35% and 50% acetonitrile) of the three fractions. Some trace CSF proteins were preferentially enriched in the two fractions, and many proteins were detected in the two-dimensional (2-D) gels of the two fractions. Among the novel proteins identified, sixty-two protein spots that represent forty-two proteins were characterized. Most of the proteins have not been annotated in any previous 2-D map of human CSF, and several have been implicated in CNS diseases. The prefractionation of CSF proteins with SPE, followed by proteomics analysis, provides a new method to explore low-abundance, disease-specific CSF proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据