期刊
FEMS MICROBIOLOGY LETTERS
卷 243, 期 1, 页码 293-301出版社
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2004.12.019
关键词
stromatolites; cyanobacteria; peptide synthetase; polyketide synthase; MALDI-TOF
类别
The cyanobacterial communities associated with stromatolites surviving in extreme habitats are a potentially rich source of bioactive secondary metabolites. We screened for the potential for production of bioactive metabolites in diverse species of cyanobacteria isolated from stromatolites in Hamelin Pool, Shark Bay, Australia. Using degenerate primer sets, putative peptide synthetase and polyketide synthase genes were detected from strains of Symploca, Leptolwigybya, Microcoleus, Pleuorocapsa, and Plectonema sp. Sequence analysis indicates the enzymes encoded by these genes may be responsible for the production of different secondary metabolites, such as hepatotoxins and antibiotics. Computer modelling was also conducted to predict the putative amino acid recognised by the unknown adenylation domain in the NRPS sequences. Mass spectral analysis also allowed the putative identification of the cyclic peptides cyanopeptolin S and 21-bromo-oscillatoxin A in two of the isolates. This is the first time evidence of secondary metabolite production has been shown in stromatolite-associated microorganisms. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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