期刊
NATURE NEUROSCIENCE
卷 8, 期 2, 页码 149-155出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nn1385
关键词
-
资金
- Howard Hughes Medical Institute Funding Source: Medline
- NIDCD NIH HHS [R01 DC000241-26, DC00241, R01 DC000241, F32 DC000241] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007739, GM07739] Funding Source: Medline
An active process in the inner ear expends energy to enhance the sensitivity and frequency selectivity of hearing. Two mechanisms have been proposed to underlie this process in the mammalian cochlea: receptor potential-based electromotility and Ca2+-driven active hair-bundle motility. To link the phenomenology of the cochlear amplifier with these cellular mechanisms, we developed an in vitro cochlear preparation from Meriones unguiculatus that affords optical access to the sensory epithelium while mimicking its in vivo environment. Acoustic and electrical stimulation elicited microphonic potentials and electrically evoked hair-bundle movement, demonstrating intact forward and reverse mechanotransduction. The mechanical responses of hair bundles from inner hair cells revealed a characteristic resonance and a compressive nonlinearity diagnostic of the active process. Blocking transduction with amiloride abolished nonlinear amplification, whereas eliminating all but the Ca2+ component of the transduction current did not. These results suggest that the Ca2+ current drives the cochlear active process, and they support the hypothesis that active hair-bundle motility underlies cochlear amplification.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据