期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 53, 期 3, 页码 671-677出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf0487506
关键词
carotenoids; deuterated vegetables; HPLC-APCI/MS; H-1 NMR
The structures of biosynthetic deuterated carotenoids in labeled vegetables were investigated: (all-E)-lutein and (all-E)-beta-carotene from spinach, and (all-E)-beta-carotene and (all-E)-alpha-carotene from carrots. The vegetables were grown hydroponically using a nutrient solution enriched with deuterium oxide (D2O) and were extracted using matrix solid-phase dispersion (MSPD). Deuterium enrichment in the carotenoid molecules was determined by liquid chromatography-mass spectrometry (LC-MS). (all-E)-Lutein and (all-E)-beta-carotene in spinach showed partial deuteration from H-2(1) to H-2(12), with the abundance maximum at H-2(5). (all-E)-beta-Carotene and (all-E)-alpha-carotene from carrots showed partial deuteration from H-2(1) to H-2(17), with the abundance maximum at H-2(11). The H-1 NMR spectra of the four deuterated carotenoids showed additional signals for all methyl groups and decreased signal intensity for the olefinic protons and the methylene protons in the ring. These differences are due to isotopic effects and are based on the substitution of protons by deuterium atoms. The deuteration was distributed randomly throughout the carotenoid molecules.
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