期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1065, 期 1, 页码 69-73出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.10.051
关键词
methacrylate monoliths; PolyHIPE; emulsion polymerisation; protein separation; polymer chromatographic supports
Poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate) monolithic supports were prepared by radical polymerisation of the continuous phase of water in oil high internal phase emulsions. Morphology of monolithic materials was studied by scanning electron microscopy and mercury intrusion porosimetry. The ratio of phase volume and the degree of crosslinking influenced the void size and pore size distribution of resulting polymers. Void sizes between 1 and 10 mum were observed and average pore sizes around 100 run. Polymers with 60, 75, 80 and 90% pore volume were prepared and even samples with highest pore volume showed good mechanical stability. They were modified to bear weak-anion exchange groups and tested on the separation of standard protein mixture containing myoglobin, conalbumine and trypsin inhibitor. Good separation was obtained in a very short time similar to the separation obtained by commercial methacrylate monoliths. However, higher dispersion was observed. Bovine serum albumin dynamic binding capacity for monolith with 90% porosity was close to 9 mg/ml. (C) 2004 Elsevier B.V. All rights reserved.
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