期刊
ANALYTICA CHIMICA ACTA
卷 530, 期 2, 页码 237-243出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2004.09.038
关键词
cyromazine; melamine; pesticide residues; vegetables; liquid chromatography; tandem mass spectrometry
A method has been developed for the sensitive and selective determination of cyromazine and its metabolite melamine in chard samples. Both compounds are small polar basic molecules, making their determination at residue levels complicated. The method involves an extraction procedure with phosphate buffer and methanol using high-speed blender, the addition of tridecafluoroheptanoic acid (TFHA) as ion-pair reagent and the injection of the five-fold diluted extract on liquid chromatography coupled to electrospray tandem mass spectrometry (LC-ESI-MS/MS). The method has been validated for chard samples, spiked at 0.05 and 0.5 mg kg(-1). Quantification was carried out by using matrix-matched standards calibration and recoveries were satisfactory, with mean values for cyromazine of 103% and 93%, and relative standard deviations lower than 7%. In the case of melamine, recoveries were 89% and 86%, with relative standard deviations lower than 13%. A limit of quantification of 0.05 mg kg(-1) was obtained for both compounds, with the limit of detection below 0.01 mg kg(-1). The method, with very little sample handling and good sensitivity, was applied to the rapid determination of low residue levels of these compounds in chards from field residue trials. All the quality controls included during the analysis were satisfactory with average recoveries of 92% and 78% for cyromazine and melamine, respectively. (C) 2004 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据