期刊
FEBS LETTERS
卷 579, 期 5, 页码 995-1000出版社
WILEY
DOI: 10.1016/j.febslet.2004.12.072
关键词
+2 codon; IF1; gene expression; codon context; aminoacyl-tRNA acceptor site; Escherichia coli
The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3A' reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutants is similar to the wild-type strain. The results do not support the longstanding hypothesis that IF1 could perform discriminatory functions while blocking the aminoacyl-tRNA acceptor site (A-site) of the ribosome. One cold-sensitive IF1 mutant shows a general overexpression, in particular at low temperatures, of both reporter genes at the protein but not mRNA level. (C) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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