期刊
ANALYTICA CHIMICA ACTA
卷 531, 期 1, 页码 1-5出版社
ELSEVIER
DOI: 10.1016/j.aca.2004.10.072
关键词
label-free; amperometric; Japanese B encephalitis vaccine; immunosensor
A label-free amperometric immunosensor for fast and sensitive assay of Japanese B encephalitis vaccine is presented. Antiserum of Japanese B encephalitis were immobilized on bilayer nano-Au/o-phenylenediamine polymer film with deposited Prussian blue as an electronic mediator on the Pt electrode. The electrochemical behavior of the biosensor was studied with Fe2+/3+ as probe on the Pt surface using cyclic voltammetry technique. The variation of amperometric response to the concentration of Japanese B encephalitis vaccine, the target antigen, was evaluated by cyclic voltammetry in PBS. The immunosensor showed a specific response to Japanese B encephalitis vaccine in the range 1.1 x 10(-8) to 1.9 x 10(-6) lg pfu/ml (pfu/ml is plaque forming unit and lg is common logarithm) with a detection limit of 6 x 10(-9) lg pfu/ml. The correlation coefficient is 0.9955. The incubation time, incubation temperature, pH, reproducibility and stability of the immunosensor were also studied. The present work supplied a promising test method for biological products. (c) 2004 Elsevier B.V. All rights reserved.
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