期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1066, 期 1-2, 页码 47-53出版社
ELSEVIER
DOI: 10.1016/j.chroma.2004.11.103
关键词
mixed mode interaction; complementary separation; two-dimensional liquid chromatography
Mixed mode stationary phases with ion-pairing reagent (acidic or basic) as integral part of hydrophobic chain offers unique selectivity, and hence, are ideal for multidimensional separations. The retention of hydrophobic components is a function of organic content, whereas that of charged species is a function of organic content, ionogenic modifier and its level in the mobile phase. Hence, by controlling the parameters influencing component retention (stationary phase and mobile phase), the selectivity of chemical components in the two-dimensional plane can be manipulated to improve the separation. A two-dimensional liquid chromatograph has been developed by coupling similar and dissimilar mixed mode stationary phases in the two dimensions. This technique has immense potential in resolving co-eluting components as the retention mechanism in the two-dimensions are complementary. However, with only part of the primary column eluent sampled into the secondary column, the technique is limited to qualitative analysis. (C) 2004 Elsevier B.V. All rights reserved.
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