期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 346, 期 3, 页码 665-676出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2004.12.010
关键词
archebacteria; bacterial flagella; cryo-electron-microscopy; Halobacterium; helical real space image reconstruction
资金
- NIBIB NIH HHS [EB001567] Funding Source: Medline
The eubacterial flagellar filament is an external, self-assembling, helical polymer similar to220 Angstrom in diameter constructed from a highly conserved monomer, flagellin, which polymerizes externally at the distal end. The archaeal filament is only similar to100 Angstrom in diameter, assembles at the proximal end and is constructed from different, glycosylated flagellins. Although the phenomenology of swimming is similar to that of eubacteria, the symmetry of the archebacterial filament is entirely different. Here, we extend our previous study on the flagellar coiled filament structure of strain R1M1 of Halobacterium salinarum. We use strain M175 of H. salinarum, which forms poly-flagellar bundles at high yield which, under conditions of relatively low ionic-strength (0.8 M versus 5 M) and low pH (similar to2.5 versus similar to6.8), form straight filaments. We demonstrated previously that a single-particle approach to helical reconstruction has many advantages over conventional Fourier-Bessel methods when dealing with variable helical symmetry and heterogeneity. We show here that when this method is applied to the ordered helical structure of the archebacterial uncoiled flagellar filament, significant extensions in resolution can be obtained readily when compared to applying traditional helical techniques. The filament population can be separated into classes of different morphologies, which may represent polymorphic states. Using cryo-negatively stained images, a resolution of similar to10-15 Angstrom has been achieved. Single alpha-helices can be fit into the reconstruction, supporting the proposed similarity of the structure to that of type IV bacterial pili. (C) 2004 Elsevier Ltd. All rights reserved.
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