期刊
JOURNAL OF CELL BIOLOGY
卷 168, 期 5, 页码 713-722出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200411126
关键词
-
类别
资金
- NIGMS NIH HHS [R01 GM059363-06, GM30758, R01 GM030758, R01 GM059363, GM06627, GM59363] Funding Source: Medline
It has been reported that nontransformed mammalian cells become arrested during G, in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547-1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurgery) does not impede cell cycle progression in HeLa cells. HeLa cells born without centrosomes, later, assemble a variable number of centrioles de novo. Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase. These, initially amorphous precentrioles become morphologically recognizable centrioles before mitosis. De novo-assembled centrioles mature (i.e., gain abilities to organize microtubules and replicate) in the next cell cycle. This maturation is not simply a time-dependent phenomenon, because de novo-formed centrioles do not mature if they are assembled in 5 phase-arrested cells. By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据