期刊
LIPIDS
卷 40, 期 3, 页码 249-257出版社
AMER OIL CHEMISTS SOC A O C S PRESS
DOI: 10.1007/s11745-005-1379-z
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Potato tuber lipoxygenase (ptLOX) has been shown to catalyze the aerobic formation of at least four major oxygenated derivatives of DHA. Two of the products-7,1 7(S)- and 10,11 7(S)-dihydro(pero)xy-DHA [7,17- and 10,17-diH(P)DHA]were formed from soybean 15-LOX-derived 17(S)-hydro(pero)xy-DHA [17(S)-H(P)DHA], whereas two novel oxylipin compounds-10(S)-hydro(pero)xy-DHA and 10,20-dihydro(pero)xy-DHA [10(S)-H(P)DHA and 10,20-diH(P)DHA, respectively]-were the major direct products of DHA oxidation by ptLOX. The reactions proceeded relatively slowly but could be stimulated by catalytic amounts of SDS. Micromolar concentrations of 10(S)-HPDHA effectively abolished the kinetic lag period of ptLOX activation. Enzymatic activity with DHA or I 7(S)-HPDHA as substrate was about 8% of that with linoleic acid-a standard natural ptLOX substrate-whereas 1 7(S)-HDHA was converted at a rate of 1 %. The enzyme was relatively unstable and quickly inactivated during the reaction with DHA on with I 7(S)-HPDHA (first-order kinetic constant of inactivation k(in) = 1.5 +/- 0.3 min(-1)), but not with 17(S)-HDHA. Both 7,17- and 10,20-diH(P)DHA were clearly products of double oxygenation catalyzed by soybean 15-LOX and/or ptLOX. Our observation that ptLOX could convert 17-HDHA to 10,17-diH(P)DHA indicates that this dihydroxylated derivative of DHA also can be formed via a double lipoxygenation mechanism.
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