期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 15, 期 5, 页码 1521-1524出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2004.12.065
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资金
- NIGMS NIH HHS [GM62159] Funding Source: Medline
Using a genetic selection we identified mutants of the M. janaschii tyrosyl-tRNA synthetase that selectively charge an amber suppressor tRNA with para-propargyloxyphenylalanine in Escherichia coli. These evolved tRNA-synthetase pairs were used to site-specifically incorporate an alkynyl group into a protein, which was subsequently conjugated with fluorescent dyes by a [3+2]-cycloaddition reaction under mild reaction conditions. (c) 2004 Elsevier Ltd. All rights reserved.
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