期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 39, 期 5, 页码 1257-1262出版社
AMER CHEMICAL SOC
DOI: 10.1021/es049234z
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We have built a high-resolution differential surface plasmon resonance (SPR) sensor for heavy metal ion detection. The sensor surface is divided into a reference and sensing areas, and the difference in the SPR angles from the two areas is detected with a quadrant cell photodetector as a differential signal. In the presence of metal ions, the differential signal changes due to specific binding of the metal ions onto the sensing area coated with properly selected peptides, which provides an accurate real-time measurement and quantification of the metal ions. Selective detection of CU2+ and Ni2+ in the ppt-ppb range was achieved by coating the sensing surface with peptides NH2Gly-Gly-His-COOH and NH2-(HiS)(6)-COOH. CU2+ in drinking water was tested using this sensor.
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