期刊
GENETICS AND MOLECULAR BIOLOGY
卷 28, 期 1, 页码 1-9出版社
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572005000100001
关键词
phage display; applications
The expression of exogenous peptides on the surface of filamentous bacteriophage was initially described by Smith in 1985. Since his first study, different molecules such as small peptides and antibodies have been displayed on coat proteins of phage, greatly expanding the applications of the technology. The past decade has seen considerable progress in the techniques and applications of phage libraries. In addition, different screening methods have allowed isolation and characterization of peptides binding to several molecules in vitro, in the context of living cells, in animals and in humans. Here we review the applications, recent innovations, and future directions of phage display technology.
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