期刊
MECHANISMS OF DEVELOPMENT
卷 122, 期 3, 页码 263-272出版社
ELSEVIER
DOI: 10.1016/j.mod.2004.11.003
关键词
Xenopus; tadpoles; mutants; husbandry; genetics; grinch; curly; bubblehead
资金
- NICHD NIH HHS [K12-HD00850, K08-HD42550] Funding Source: Medline
Xenopus tropicalis offers the potential for genetic analysis in an amphibian. In order to take advantage of this potential, we have been inbreeding strains of frogs for future mutagenesis. While inbreeding a population of Nigerian frogs, we identified three mutations in the genetic background of this strain. These mutations are all recessive embryonic lethals. We show that multigenerational mutant analysis is feasible and demonstrate that mutations can be identified, propagated, and readily characterized using hybrid, dihybrid, and even trihybrid crosses. In addition. we are optimizing conditions to raise frogs rapidly and present our protocols for X. tropicalis husbandry. We find that males mature faster than females (currently 4 versus 6 months to sexual maturity). Here we document our progress in developing X. tropicalis as a genetic model organism and demonstrate the utility of the frog to Study the genetics of early vertebrate development. (c) 2004 Published by Elsevier Ireland Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据