期刊
FEMS MICROBIOLOGY ECOLOGY
卷 52, 期 1, 页码 101-114出版社
OXFORD UNIV PRESS
DOI: 10.1016/j.femsec.2004.10.015
关键词
haemodialysis water; 16S rRNA gene; biofilm; bacterial diversity
类别
Water used to prepare haemodialysis fluid is not sterile, and its microbiological control is important for the prevention of haemodialysis-associated illness. Bacterial populations inhabiting a distribution system for haemodialysis water were studied over an 18-month period. 203 planktonic bacteria isolated on R2A medium were identified by restriction analysis and sequencing of 16S rRNA gene. A diverse bacterial community was detected, containing predominantly Gram-negative members of the Alphaproteobacteria and Betaproteobacteria, as well as representatives of the genus Mycobacterium. Ecological and clinical consequences are discussed: bacteria from the genera Novosphingobium, Pseudomonas and Sphingomonas have been described in the build-up of biofilms, and others like Acinetobacter, Mycobacterium or Brevibacterium may represent a health risk to patients under haemodialysis treatment. 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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