期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 3, 期 2, 页码 141-155出版社
WILEY
DOI: 10.1111/j.1467-7652.2004.00113.x
关键词
chloroplast transformation; co-transformation; linked transgenes; marker gene excision; metabolic engineering; multigene manipulation; polyproteins; pyramiding; retransformation; stacking
One of the major technical hurdles impeding the advance of plant genetic engineering and biotechnology is the fact that the expression or manipulation of multiple genes in plants is still difficult to achieve. Although a small proportion of commercial genetically modified (GM) crops present 'stacked' or 'pyramided' traits, only a handful of products have been developed by introducing three or more novel genes. On the research front, a variety of conventional and more novel methods have been employed to introduce multiple genes into plants, but all techniques suffer from certain drawbacks. In this review, the potential and problems of these various techniques and strategies are discussed, and the prospects for improving these technologies in the future are presented.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据