Nonomuraea kuesteri sp nov.

A Gram-positive, aerobic, non-motile actinomycete, strain GW 14-1925(T), that formed branched substrate and aerial mycelium was studied using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strain GW 14-1925(T) was shown to belong to the genus Nonomuraea, being most closely related to Nonomuraea longicatena (97(.)9%), Nonomuraea turkmeniaca (98(.)9%), Nonomuraea helvata (98(.)6%), Nonomuraea polychroma (98(.)5%), Nonomuraea salmonaea (98(.)3%), Nonomuraea roseoviolacea subsp. roseoviolacea (98(.)1%) and Nonomuraea roseoviolacea subsp. carminata (97(.)7%). The 16S rRNA gene sequence similarity to other Nonomuraea species was < 97(.)5%. Chemotaxonomic data [major menaquinones of the MK-9 series with minor amounts of MK-8(H-4); major polar lipids of phospholipid type IV; fatty acids with major amounts of iso- and anteiso- and 10-methyl-branched fatty acids in combination with iso-branched 2-hydroxy fatty acids] supported allocation of the strain to the genus Nonomuraea. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW 14-1925(T) from closely related species; thus, GW 14-1925(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea kuesteri sp. nov. is proposed, with GW 14-1925(T) (= DSM 44753(T) = NRRL B-24325(T)) as the type strain.