期刊
BIOTECHNOLOGY LETTERS
卷 27, 期 5, 页码 313-316出版社
SPRINGER
DOI: 10.1007/s10529-005-0685-5
关键词
alkaline cellulase; alkalophilic; Bacillus sp; characterization; purification
An alkaline cellulase from Bacillus sp. HSH-810 was purified 8.7-fold with a 30% yield and a specific activity of 71 U mg(-1) protein. It was optimally active at pH 10 and 50 degrees C and was stable from pH 6 to 10 with more than 60% activity remaining after heating at 60 degrees C for 60 min. The molecular mass of cellulase was 80 kDa. It was inhibited by 50% by Fe3+ (1 mM) and Mn2+(0.1 mM) but was relatively insensitive to Hg2+ and Pb2+ at 1 mM.
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