Correction of accurate mass measurement for target compound verification by quadrupole time-of-flight mass spectrometry

The aim of this week is to evaluate quadrupole/ time-of-flight (QTOF) mass spectrometry for simultaneous measurement of accurate mass and quantification of a target by using a stable isotopically labeled internal standard. Mixtures of caffeine and C-13(3)-caffeine (internal standard) at different concentration ratios were analyzed by capillary HPLC/QTOF. A calibration plot for quantification is linear over a factor of 20. Without invoking any correction scheme, the mass accuracy seriously degraded when the ratio of the mass standard to the test compound was not unity. The accuracy could be restored to approximately 2 ppm by using a quadratic function to correct the measured mass as a function of the measured signal ratio of target and internal calibrant. (J Am Soc Mass Spectrorn 2005,16, 406-408) (C) 2004 American Society for Mass Spectrometry.