期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 162, 期 3, 页码 355-361出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2004.07.015
关键词
carbon dioxide; culture vessel microenvironment; ethytene; hyperhydricity; Scrophularia yoshimurae
An effective procedure for obtaining healthy shoots from nodal segments of Scrophularia yoshimurae is described. Nodal segments cultured on Murashige and Skoog's (MS) basal medium supplemented with 1.0 mg L-1 benzyladenine (BA) and 0.2 mg L-1 alpha-naphthateneacetic acid (NAA) induced multiple shoots in conical. flasks that differed in the way they were closed and seated. Hermitically seated culture vessels resulted in high hyperhydricity/vitrification. High concentrations of ethylene and CO2 were found to accumulate in these vessels. The hyperhydricity of the shoot cultures could be decreased by progressively ventilating the vessels. Exchange of gases was achieved by removing the Parafilm sealing without affecting sterility. This reduced the hyperhydricity rate and gave a good recovery of vitrified shoots, but resulted in decreased proliferation and a dehydration of proliferating nodal segments and the culture medium. The best number of normal shoots was observed when the parafilm was removed for gaseous exchange after four weeks of culture incubation. The result's show that hyperhydricity in shoot cultures of S. yoshimurae could be prevented by sufficient gas exchange during culture. (c) 2004 Elsevier GmbH. All rights reserved.
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