期刊
JOURNAL OF CELL SCIENCE
卷 118, 期 5, 页码 873-887出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.01634
关键词
cadherin; TGF-beta; epithelium-to-mesenchyme transition; motility
类别
资金
- NCRR NIH HHS [P20-RR018759] Funding Source: Medline
- NIDCR NIH HHS [R01-DE12308] Funding Source: Medline
- NIGMS NIH HHS [R01-GM51188] Funding Source: Medline
Epithelium-to-mesenchyme transitions (EMTs) are characterized by morphological and behavioral changes in cells. During an EMT, E-cadherin is downregulated while N-cadherin is upregulated. The goal of this study was to understand the role cadherin switching plays in EMT using a classical model system: transforming growth factor Pi (TGF-beta 1)-mediated EMT in mammary epithelial cells. We showed that stress fibers and focal adhesions are increased, and cell-cell junctions are decreased in response to TGF-beta 1. Moreover, these changes were reversible upon removal of TGF- I. Downregulation of E-cadherin and upregulation of N-cadherin were both transcriptional. Neither experimental knockdown nor experimental overexpression of N-cadherin interfered with the morphological changes. In addition, the morphological changes associated with EMT preceded the downregulation of E-cadherin. Interestingly, TGF-beta 1-induced motility in N-cadherin-knockdown cells was significantly reduced. Together, these data suggest that cadherin switching is necessary for increased motility but is not required for the morphological changes that accompany EMT.
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