期刊
APPLIED OPTICS
卷 52, 期 1, 页码 A300-A309出版社
OPTICAL SOC AMER
DOI: 10.1364/AO.52.00A300
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资金
- National Science Foundation [CBET-0756005]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0756005] Funding Source: National Science Foundation
Holographic optical coherence imaging is an en face form of optical coherence tomography that uses low-coherence digital holography as a coherence gate to select light from a chosen depth inside scattering tissue. By acquiring successive holograms at a high camera frame rate at a fixed depth, dynamic speckle provides information concerning dynamic light scattering from intracellular motility. Motility contrast imaging (MCI) uses living motion as a label-free and functional biomarker. MCI provides a new form of viability assay and also is applicable for proliferation and cytotoxicity assays. The results presented here demonstrate that low-coherence digital holography can extract viability information from biologically relevant three-dimensional (3D) tissue based on multicellular tumor spheroids by moving beyond the format of two-dimensional cell culture used for conventional high-content analysis. This paper also demonstrates the use of MCI for chemosensitivity assays on tumor exgrafts of excised ovarian cancer tumors responding to standard-of-care cisplatin chemotherapy. This ex vivo application extends the applicability of MCI beyond 3D tissue culture grown in vitro. (C) 2012 Optical Society of America
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