期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 149, 期 3, 页码 227-234出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2004.10.006
关键词
election tomography; Cryo-EM; automated data acquisition; reconstruction software
Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. 'TOM software toolbox' integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly Unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications. (C) 2004 Elsevier Inc. All rights reserved.
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