期刊
JOURNAL OF BACTERIOLOGY
卷 187, 期 6, 页码 2058-2065出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.187.6.2058-2065.2005
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资金
- NIGMS NIH HHS [R01 GM059690, GM59690] Funding Source: Medline
The bacterium Vibrio fischeri requires bacterial motility to initiate colonization of the Hawaiian squid Euprymna scolopes. Once colonized, however, the bacterial population becomes largely unflagellated. To understand environmental influences on V. fischeri motility, we investigated migration of this organism in tryptone-based soft agar media supplemented with different salts. We found that optimal migration required divalent cations and, in particular, Me2+. At concentrations naturally present in seawater, Mg2+ improved migration without altering the growth rate of the cells. Transmission electron microscopy and Western blot experiments suggested that Mg2+ addition enhanced flagellation, at least in part through an effect on the steady-state levels of flagellin protein.
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