期刊
INTERNATIONAL DAIRY JOURNAL
卷 15, 期 3, 页码 219-229出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.idairyj.2004.07.004
关键词
alpha-lactalbumin; proteolysis; fragments; assembly; amyloid fibrils
Bovine alpha-lactalbumin (alpha-LA) (10 g L-1) was incubated with a protease from Bacillus licheniformis at pH 7.5 and 50 degreesC. The reaction was biphasic consisting of an initial hydrolysis of intact alpha-LA and formation of dimers from large hydrolysis products within 60 min followed by aggregation of dimers into aggregates of 500 kDa. The aggregates consisted primarily of fibrillar strands with a diameter of 5 nm. Formation of these strands was accompanied by a change in secondary structure towards higher beta-sheet content and strong binding of thioflavin, features shared with amyloidal fibrils. The main components in these fibrils were fragments of 8.8 and 9.8 kDa shown to occur in a monomer-dimer equilibrium. These fragments were identified and a molecular mechanism involving side-by-side assembly of dimers of these fragments into fibrils is proposed. (C) 2004 Elsevier Ltd. All rights reserved.
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