期刊
CHEMISTRY AND PHYSICS OF LIPIDS
卷 134, 期 1, 页码 1-20出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2004.12.003
关键词
levuglandin; isolevuglandin; cyclooxygenase; oxidative stress; lipid oxidation
资金
- NHLBI NIH HHS [HL53315] Funding Source: Medline
- NIGMS NIH HHS [GM21249] Funding Source: Medline
The cyclooxygenase (COX) pathway generates enantiomerically pure levuglandin (LG) E-2 by a rearrangement of the prostaglandin (PG) endo-peroxide PGH(2). The isoprostane pathway generates racemic LGE(2) together with stereoisamers, designated collectively as isoLGE(2), through free radical-induced lipid oxidation. Within seconds, both LGs and isoLGs are rapidly sequestered by protein adduction. In theory, the diastereomeric purity of LGE(2)-protein adduct-derived lysyl lactams can reveal the relative contributions of the COX and isoprostane pathways to LGE(2) stereoisomer production in vivo. Notably, however, the detection of LGE(2)-protein adducts does not provide a basis for inferring their formation through the isoprostane pathway in vivo unless the COX pathway can be rigorously excluded. In contrast, LGE(2) structural isomers, designated collectively as iso[n]LGE(2)s, are produced exclusively through the isoprostane pathway. Immunoassays that selectively recognize iso[n]LGE(2)-protein adducts are the only tools available to unambiguously detect and quantify the production of isolevuglandins in vivo through free radical-induced oxidation of arachidonates. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据