期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 98, 期 23, 页码 9527-9544出版社
SPRINGER
DOI: 10.1007/s00253-014-6142-4
关键词
Lignin; Degradation; Prokaryotes; Screening; Culture-dependent; Culture-independent; Phylogenetic distribution
资金
- French Environment and Energy Agency, ADEME [13 06 C 0068]
A number of prokaryotes actively contribute to lignin degradation in nature and their activity could be of interest for many applications including the production of biogas/biofuel from lignocellulosic biomass and biopulping. This review compares the reliability and efficiency of the culture-dependent screening methods currently used for the isolation of ligninolytic prokaryotes. Isolated prokaryotes exhibiting lignin-degrading potential are presented according to their phylogenetic groups. With the development of bioinformatics, culture-independent techniques are emerging that allow larger-scale data mining for ligninolytic prokaryotic functions but today, these techniques still have some limits. In this work, two phylogenetic affiliations of isolated prokaryotes exhibiting ligninolytic potential and laccase-encoding prokaryotes were determined on the basis of 16S rDNA sequences, providing a comparative view of results obtained by the two types of screening techniques. The combination of laboratory culture and bioinformatics approaches is a promising way to explore lignin-degrading prokaryotes.
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