期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 98, 期 14, 页码 6353-6364出版社
SPRINGER
DOI: 10.1007/s00253-014-5678-7
关键词
Peanut allergy; Immunomodulation; Oral tolerance; Lactococcus lactis
资金
- National Natural Science Foundation of China [31200691]
- National Science Fund for Distinguished Young Scholars [31125021]
- National Science & Technology Pillar Program in the Twelfth Five-year Plan Period [2013BAD18B01, 2013BAD18B02, 2012BAD28B07, 2012BAD28B08]
- 111 project [B07029, IRT1249]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Peanut allergy (PNA) has becoming a non-negligible health concern worldwide. Thus far, allergen-specific immunotherapy aimed at inducing mucosal tolerance has widely been regarded as a major management strategy for PNA. The safety profiles and the intrinsic probiotic properties of lactic acid bacteria (LAB) render them attractive delivery vehicles for mucosal vaccines. In the present study, we exploited genetically modified Lactococcus lactis to produce peanut allergen Ara h 2 via different protein-targeting systems and their immunomodulatory potency for allergic immune responses in mice were investigated. By comparison with the strain expressing the cytoplasmic form of Ara h 2 (LL1), the strains expressing the secreted and anchored forms of Ara h 2 (LL2 and LL3) were more potent in redirecting a Th2-polarized to a non-allergic Th1 immune responses. Induction of SIgA and regulatory T cells were also observed at the local levels by orally administration of recombinant L. lactis. Our results indicate that allergen-producing L. lactis strains modulated allergic immune responses and may be developed as promising mucosal vaccines for managing allergic diseases.
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