期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 35, 期 3, 页码 852-858出版社
WILEY-BLACKWELL
DOI: 10.1002/eji.200425423
关键词
ATP; cytokines; TNF-alpha; Il-10; blood
类别
In vitro studies suggest that extracellular nucleotides and nucleosides may be important regulators of inflammatory and immune responses. Most studies with adenosine 5'-triphosphate (ATP) have been performed in cell lines, which are remote from the human situation. The purpose of the present study was to determine the effects of ATP on TNF-alpha, IL-6 and IL-10 release in stimulated whole blood. Blood samples were drawn from healthy volunteers and incubated with ATP and lipopolysaccharide (LPS) + phytohemagglutinin (PHA) for 24 h. Contrary to expectations, ATP at 100 mu M and 300 mu M induced a reduction in TNF-alpha secretion by 32 +/- 8% (mean +/- SEM) and 65 +/- 4%, respectively. Furthermore, these ATP concentrations induced an increase in IL-10 secretion by 48 +/- 5% and 62 +/- 7% in whole blood. The ATP analogue adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S) and adenosine 5'-diphosphate (ADP) also inhibited TNF-alpha release, but only ADP showed a stimulatory effect on IL-10. Co-treatment with adenosine deaminase did not reverse the ATP effect on TNF-alpha and IL-10. These results show, for the first time, that ATP inhibits the inflammatory response in stimulated whole blood as indicated by inhibition of TNF-alpha and stimulation of IL-10 release and that this effect is predominantly mediated by ATP and not by adenosine.
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