期刊
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
卷 90, 期 3, 页码 1752-1759出版社
ENDOCRINE SOC
DOI: 10.1210/jc.2004-1092
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资金
- NCRR NIH HHS [5MO1-RR-00056] Funding Source: Medline
- NIBIB NIH HHS [EB-01975] Funding Source: Medline
- NIDDK NIH HHS [P30-DK-46204, DK-60555-02] Funding Source: Medline
Insulin-stimulated glucose transport in skeletal muscle is regarded as a key determinant of insulin sensitivity, yet isolation of this step for quantification in human studies is a methodological challenge. One notable approach is physiological modeling of dynamic positron emission tomography ( PET) imaging using 2-[18-fluoro] 2-deoxyglucose ([F-18] FDG); however, this has a potential limitation in that deoxyglucose undergoes phosphorylation subsequent to transport, complicating separate estimations of these steps. In the current study we explored the use of dynamic PET imaging of [C-11] 3-O-methylglucose ([C-11] 3-OMG), a glucose analog that is limited to bidirectional glucose transport. Seventeen lean healthy volunteers with normal insulin sensitivity participated; eight had imaging during basal conditions, and nine had imaging during euglycemic insulin infusion at 30 mU/ min center dot m(2). Dynamic PET imaging of calf muscles was conducted for 90 min after the injection of [C-11] 3-OMG. Spectral analysis of tissue activity indicated that a model configuration of two reversible compartments gave the strongest statistical fit to the kinetic pattern. Accordingly, and consistent with the structure of a model previously used for [F-18] FDG, a two-compartment model was applied. Consistent with prior [F-18] FDG findings, insulin was found to have minimal effect on the rate constant for movement of [C-11] 3-OMG from plasma to tissue interstitium. However, during insulin infusion, a robust and highly significant increase was observed in the kinetics of inward glucose transport; this and the estimated tissue distribution volume for [C-11] 3-OMG increased 6-fold compared with basal conditions. We conclude that dynamic PET imaging of [C-11] 3-OMG offers a novel quantitative approach that is both chemically specific and tissue specific for in vivo assessment of glucose transport in human skeletal muscle.
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